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KMID : 0665220090220010103
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Korean Journal of Food and Nutrition
2009 Volume.22 No. 1 p.103 ~ p.109
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Isolation and Optimal Producing Conditions of Broad Spectrum Antibiotics from Streptomyces sp. Y-88
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Bang Byung-Ho
Jeong Eun-Ja
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Abstract
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In order to isolate antibiotic producing microorganisms, several actinomycetes were isolated from soil samples. The aerial hyphae of Y-88 strain were gray in color with tree types. Under the microscopic examination, the Y-88 isolate formed a spiral aerial spore mass with a smooth surface and a rectiflexibilis type of spore chain. Y-88 utilized glucose, fructose, arabinose, and sucrose, but not rhamnose, raffinose, mannitol, or inositol. In addition, Y-88 produced melanin on the tyrosine agar and the strain could utilize L-valine, L-phenylalanine, and L-hydroxyproline. Based on these results and the cultural and physiological characteristics described in the Bergey’s Manual, the Actinomycetes, Y-88, was identificated as a Streptomyces species. The optimum medium conditions for this antibiotic producing Streptomyces sp. Y-88 was 1.6% soluble starch, 0.6% glucose, 0.6% beef extract, 0.01% , 0.01% , and 0.01% at an initial pH of 4.0, and 25.
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KEYWORD
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Actinomycetes, antibiotic, broad spectrum antibiotic, cultural conditions, identification
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